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Alexa Fluor® 488 anti-human TCR α/β
产品名称:
Alexa Fluor® 488 anti-human TCR α/β
产品类别:
抗体
产品编号:
306711
产品应用:
306711
[价格]
规格 价格 库存
25tests ¥ 1488 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone. ?Other anti-CD3 clones may compete out the binding of IP26. ?

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. (FC)
  2. Joseph A, et al. 2008. J. Virol. 82:3078. (FC) PubMed
  3. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
Product Citations
  1. Holm D, et al. 2013. Immunobiology. 218:408. PubMed
  2. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  3. Panwar B, et al. 2021. Genome Res. 31:659. PubMed
  4. Massafra V, et al. 2021. J Immunol. 207:493. PubMed
  5. Kula T, et al. 2019. Cell. 178:1016. PubMed
  6. Ahmed R, et al. 2021. STAR Protoc. 2:100925. PubMed
  7. Japp AS, et al. 2021. Cell. 184(3):827-839.e14. PubMed
  8. Singh A, et al. 2020. Cell Rep. 32:108153. PubMed
RRID
AB_528966 (BioLegend Cat. No. 306711) AB_528967 (BioLegend Cat. No. 306712)

Antigen Details

Structure
Ig superfamily, with CD3 forms CD3/TCR complex
Distribution

T cells, thymocytes

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound to MHC
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Marchalonis J, et al. 2002. J. Mol. Recognit. 15:260.

Gene ID
6955 View all products for this Gene ID 6957 View all products for this Gene ID
UniProt
View information about TCR alpha/beta on UniProt.org

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