Alexa Fluor® 488 anti-mouse CD74 (CLIP) Antibo-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1836	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC -?Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. For immunohistochemistry, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Blue Laser (488 nm)

Application Notes

This clone is also known as In1 and In-1. Additional reported applications (for the relevant formats) include: immunohistochemistry¹, immunoprecipitation³, and western blotting⁴.

Application References (PubMed link indicates BioLegend citation)

Momburg F, et al. 1986. J. Immunol. 136:940. (IHC)
Takahashi K, et al. 2009. Respir. Res. 10:33. (FC)
Meza-Romero R, et al. 2014. J. Immunol. 192:4164. (IP)
Heinrichs D, et al. 2011. Proc. Natl. Acad. Sci. USA 108:17444. (WB)

Product Citations

Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
Dani N, et al. 2021. Cell. 184(11):3056-3074.e21. PubMed

RRID

AB_2750325 (BioLegend Cat. No. 151005) AB_2750326 (BioLegend Cat. No. 151006)

Antigen Details

Structure: 33.5 kD and homotrimer.
Distribution: B-cells, monocytes/macrophages, dendritic cells, activated T-cells, and activated endothelial/epithelial cells.
Function: MHC class II chaperone, MHC class II molecule sorting, and peptide loading.
Interaction: MHC class II molecules and peptides.
Ligand/Receptor: Macrophage migration inhibitory factor (MIF).
Cell Type: B cells, Dendritic cells, Endothelial cells, Epithelial cells, Macrophages, Monocytes, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, MHC Antigens
Antigen References: 1. Herrero L, et al. 2013. Arthritis Rheum. 65:2724.
2. Geneve L, et al. 2012. Int. Immunol. 24:645.
3. Sille FC, et al. 2011. Immunol Lett. 139:33.
4. Shih NY, et al. 1995. Mol. Immunol. 32:643.
5. Shi X, et al. 2006. Immunity 25:595.
6. Starlets D, et al. 2006. Blood 107:4807.
Gene ID: 16149 View all products for this Gene ID
UniProt: View information about CD74 on UniProt.org

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