热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
PE/Cyanine7 anti-mouse/human CD44 Antibody
产品名称：
PE/Cyanine7 anti-mouse/human CD44 Antibody
产品类别：
抗体
产品编号：
103029
产品应用：
103029
[价格]
规格 价格 库存
25µg ￥ 1128 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for?immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, in vivo inhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  23. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  24. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Furrer R, et al. 2021. Sci Adv. 7:eabi4852. PubMed
  2. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  3. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  4. Gerber AN, et al. 2021. Cell Rep. 37:109816. PubMed
  5. Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed
  6. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  7. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  8. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  9. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  10. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
  11. Wabitsch S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1166. PubMed
  12. He X, et al. 2021. Cell Rep. 36:109674. PubMed
  13. Wang D, et al. 2018. Immunity. 48:659. PubMed
  14. Zhang P et al. 2019. Cell Rep. 27(11):3117-3123 . PubMed
  15. Aldon Y, et al. 2020. J Immunol. 204:903. PubMed
  16. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
  17. Almeida D, et al. 2010. J Immunol. 185:1927. PubMed
  18. del Rio ML, et al. 2021. Transl Res. Online ahead of print.. PubMed
  19. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  20. Feizi N, et al. 2021. Cell Death Dis. 12:1026. PubMed
  21. Pal D et al. 2017. eLife. 6 pii: e21615. PubMed
  22. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  23. Gotoh K et al. 2018. Cell reports. 25(7):1800-1815 . PubMed
  24. Gondek D, et al. 2012. J Immunol. 189:2441. PubMed
  25. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  26. Li Y, et al. 2020. Nat Commun. 11:2781. PubMed
  27. Zhan CS, et al. 2020. FASEB J. 14006:34. PubMed
  28. LaMothe RA et al. 2018. Front Immunol. 0.570138889 . PubMed
  29. Bolinger B, et al. 2013. J Immunol. 190:4162. PubMed
  30. Kersh A, et al. 2014. J Immunol. 193:4429. PubMed
  31. Han Y, et al. 2019. Am J Cancer Res. 9:800. PubMed
  32. Ueda R, et al. 2009. Clin Cancer Res. 5.215972222. PubMed
  33. Ruf B, et al. 2021. Cancer Immunol Res. 9:1024. PubMed
  34. Stewart JM, et al. 2020. ACS Biomater Sci Eng. 6:5941. PubMed
  35. Nanou A, et al. 2020. Haematologica. . PubMed
  36. Luo Y et al. 2019. Cell reports. 26(7):1869-1879 . PubMed
  37. Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed
  38. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  39. Ruer-Laventie J, et al. 2020. Bio Protoc. e3531:10. PubMed
  40. Oladiran O, et al. 2021. J Neuroinflammation. 77:18. PubMed
  41. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  42. Ise W, et al. 2018. Immunity. 48:702. PubMed
  43. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  44. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  45. Sun L, et al. 2021. Cell Reports. 35(9):109188. PubMed
  46. Vishnoi M, et al. 2015. Sci Rep. 5: 17533. PubMed
  47. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  48. Wang D, et al. 2020. J Cell Mol Med. 24:4212. PubMed
  49. Alvim RG, et al. 2021. Molecules. 26:. PubMed
  50. Zhang Q, et al. 2022. Nat Commun. 13:2406. PubMed
  51. Anger-Góra N, et al. 2021. Oncol Lett. 22:582. PubMed
  52. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  53. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  54. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  55. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  56. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  57. Chulkina M, et al. 2017. Eur J Pharmacol. 10.1016/j.ejphar.2017.06.033. PubMed
  58. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  59. Jeljeli M, et al. 2021. Front Immunol. 12:670776. PubMed
  60. Jing Y, et al. 2021. Front Immunol. 12:651860. PubMed
  61. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  62. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  63. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  64. Limbach K, et al. 2017. Malar J. 10.1186/s12936-017-1911-z. PubMed
  65. Yomtoubian S, et al. 2020. Cell Reports. 30(3):755-770.e6.. PubMed
  66. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  67. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  68. Jeon Y, et al. 2015. PLoS One. 10: e0139845. PubMed
  69. Xu W, et al. 2019. Immunity. 51(5):826-839.e5. PubMed
  70. Hendrikx S et al. 2019. Cell reports. 26(5):1227-1241 . PubMed
  71. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  72. Skirecki T, et al. 2020. JCI Insight. 5:. PubMed
  73. Wang X, et al. 2022. Elife. 11:. PubMed
  74. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  75. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  76. Kitagawa K, et al. 2014. Mol Cell Biol. 34:2732. PubMed
  77. Castillo–Azofeifa D, et al. 2019. EMBO J. 38:e99984. PubMed
  78. Belogortseva N, et al. 2017. PLoS One.. 10.1371/journal.pone.0182825. PubMed
  79. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  80. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  81. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  82. Pattabiraman D, et al. 2016. Science. 351: aad3680. PubMed
  83. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  84. Pan Y, et al. 2021. NPJ Vaccines. 6:1. PubMed
  85. Aihara E, et al. 2015. Sci Rep. 5:17185. PubMed
  86. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
RRID
AB_830786 (BioLegend Cat. No. 103029) AB_830787 (BioLegend Cat. No. 103030)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线