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首页 > 抗体蛋白 > 抗体
Pacific Blue™ anti-mouse/human CD44 Antibody
产品名称：
Pacific Blue™ anti-mouse/human CD44 Antibody
产品类别：
抗体
产品编号：
103019
产品应用：
103019
[价格]
规格 价格 库存
25µg ￥ 1128 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD44 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for?immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, in vivo inhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
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  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
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  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
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  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  23. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  24. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Dieudé M, et al. 2015. Sci Transl Med. 7: 318ra200. PubMed
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  11. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  12. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  13. Aqel SI, et al. 2021. JCI Insight. 6:e142376. PubMed
  14. Dangi T, et al. 2021. J Clin Invest. :e151969. PubMed
  15. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
  16. Michel M, et al. 2012. Proc Natl Acad Sci U S A. 109:17459. PubMed
  17. Burgos-Ojeda D, et al. 2015. Mol Cancer Ther. 14: 1717 - 1727. PubMed
  18. Sckisel G, et al. 2017. J Immunother Cancer . 10.1186/s40425-017-0235-4. PubMed
  19. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  20. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  21. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  22. Khatun A, et al. 2021. J Exp Med. 218:. PubMed
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  24. Jena P, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.04.019. PubMed
  25. Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed
  26. Wagle MV, et al. 2021. Nat Commun. 12:2782. PubMed
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  40. Chang P, et al. 2012. J Immunol. 189: 701. PubMed
  41. Resch TK, et al. 2018. Sci Rep. 0.722916667. PubMed
  42. Yabas M, et al. 2011. PLoS One. 6:e26440. PubMed
  43. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  44. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  45. Teh C, et al. 2010. Proc Natl Acad Sci U S A. 107:14709. PubMed
  46. Kaji T, et al. 2016. Int Immunol. 28: 267 - 282. PubMed
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RRID
AB_493682 (BioLegend Cat. No. 103019) AB_493683 (BioLegend Cat. No. 103020)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

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