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Alexa Fluor® 488 anti-mouse/human CD44 Antibod
产品名称：
Alexa Fluor® 488 anti-mouse/human CD44 Antibod
产品类别：
抗体
产品编号：
103015
产品应用：
103015
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
SB -?Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for?immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, in vivo inhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  23. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  24. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Porat-Shliom N, et al. 2013. PLoS One. 25:81897. PubMed
  2. Chai Y, et al. 2016. PLoS One. 11: 0162853. PubMed
  3. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  4. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  5. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  6. Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
  7. Potluri HK, et al. 2022. J Immunother Cancer. 10:. PubMed
  8. Rivkin I, et al. 2010. Biomaterials. 31:7106. PubMed
  9. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  10. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  11. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  12. Wang Y, et al. 2019. Cell. 179:1144. PubMed
  13. García-Bonilla M, et al. 2020. Stem Cell Res Ther. 11:121. PubMed
  14. Howe EN, et al. 2020. Nat Commun. 2.553472222. PubMed
  15. Tran T, et al. 2015. Sci Rep. 5:16632. PubMed
  16. Watanabe M, et al. 2020. Nat Commun. 4.808333333. PubMed
  17. Su X, et al. 2016. Oncogene. 10.1038/onc.2016.388. PubMed
  18. Palao N, et al. 2022. Int J Biol Sci. 18:5873. PubMed
  19. Hamaidi I, et al. 2020. Cell Metabolism. 32(3):420-436.e12. PubMed
  20. Bachar G, et al. 2011. Biomaterials. 32:4840. PubMed
  21. Han X, et al. 2015. J Control Release. 197:29. PubMed
  22. Mullenders J, et al. 2019. Proc Natl Acad Sci U S A. 116:4567. PubMed
  23. Sen U, Shenoy S, and Bose B. 2017. Cell Biol Int. 10.1002/cbin.10830. PubMed
  24. Morein D, et al. 2021. Cells. 10: . PubMed
  25. Rui J, et al. 2021. Nat Commun. 12:5074. PubMed
  26. Lopes N, et al. 2022. Elife. 11:. PubMed
  27. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  28. Yamada K, et al. 2016. Cancer Res . 76: 4283 - 4292. PubMed
  29. Bouchard G, et al. 2022. Cancer Res. 82:648. PubMed
  30. Sasaki N, et al. 2016. Proc Natl Acad Sci U S A. 113: E5399 - E5407. PubMed
  31. Hao J, et al. 2009. J Cell Biol. 184:451. PubMed
  32. Katsura Y, et al. 2019. Cancers (Basel). 11:. PubMed
RRID
AB_493678 (BioLegend Cat. No. 103015) AB_493679 (BioLegend Cat. No. 103016)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

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