Ultra-LEAF? Purified anti-mouse CD254 (TRANCE, RAN-上海雅吉生物科技有限公司

规格	价格	库存
5mg	￥ 6468	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

NSO-derived recombinant mouse TRANCE

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, WB, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The IK22/5 antibody has been reported to block the binding of RANK ligand-Fc to RANK.?Additional reported applications (for the relevant formats) include: immunoprecipitation², Western blotting², and blocking of ligand binding^1,2,4. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 510009-510014).

Application References (PubMed link indicates BioLegend citation)

Miyahira Y, et al. 2003. J. Immunol. 171:6344. (Block)
Personal communication (Block IP WB)
Gao Y, et al. 2007. J. Clin. Invest. 117:122.
Kamijo S, et al. 2006. Biochem. Biophys. Res. Commun. 347:124. (Block)
Fionda C, et al. 2007. J. Immunol. 178:4039.
Haslam SZ, et al. 2008. Endocrinology. 149:2098. (Block) PubMed

RRID

AB_2832814 (BioLegend Cat. No. 510009) AB_2832815 (BioLegend Cat. No. 510010) AB_2832816 (BioLegend Cat. No. 510011) AB_2832817 (BioLegend Cat. No. 510012) AB_2832818 (BioLegend Cat. No. 510013) AB_2876738 (BioLegend Cat. No. 510014)

Antigen Details

Structure: TNF superfamily; 19 kD (Mammalian)
Distribution: Activated T cells, osteoclasts
Function: Regulates T and dendritic cell interactions, and osteoclast differentiation
Ligand/Receptor: RANK, OPG
Bioactivity: Activates JNK and NF-κB; involved in bone resorption; induces the expression of IL-1, IL-6, IL-12, IL-15 in dendritic cells
Cell Sources: Activated T cells, myeloid bone marrow precursors, stromal fibroblasts, synovial cells, osteoblasts
Cell Targets: Dendritic cells, osteoclasts
Receptors: ODFR, ODAR, TRANCE-R
Cell Type: Osteoclasts, T cells
Biology Area: Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Josien R, et al. 1999. J. Immunol. 162:2562.
3. Takayanagi H, et al. 2000. Nature 408:600.
4. Wong B, et al. 1999. Mol. Cell. 4:1041.
Regulation: Type II integral membrane protein processed by TACE for secretion; upregulated upon TCR engagement
Gene ID: 21943 View all products for this Gene ID
UniProt: View information about CD254 on UniProt.org

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