PE anti-mouse CD254 (TRANCE, RANKL) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 2340	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

NSO-derived recombinant mouse TRANCE

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric analysis, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The IK22/5 antibody has been reported to block the binding of RANK ligand-Fc to RANK.?Additional reported applications (for the relevant formats) include: immunoprecipitation², Western blotting², and blocking of ligand binding^1,2,4. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 510009-510014).

Application References (PubMed link indicates BioLegend citation)

Miyahira Y, et al. 2003. J. Immunol. 171:6344. (Block)
Personal communication (Block IP WB)
Gao Y, et al. 2007. J. Clin. Invest. 117:122.
Kamijo S, et al. 2006. Biochem. Biophys. Res. Commun. 347:124. (Block)
Fionda C, et al. 2007. J. Immunol. 178:4039.
Haslam SZ, et al. 2008. Endocrinology. 149:2098. (Block) PubMed

Product Citations

Dai Y, et al. 2013. Mol Cancer Ther. 12:878. PubMed
Hinks TSC, et al. 2019. Cell Rep. 28:3249. PubMed
Dar HY, et al. 2018. Sci Rep. 8:2503. PubMed
Dar HY, et al. 2018. Bone Rep. 8:46. PubMed
Sapra L, et al. 2021. Sci Rep. 11:1807. PubMed
Ding P, et al. 2022. Bone Res. 10:42. PubMed
Gao Y, et al. 2007. J Clin Invest. 117:122. PubMed
Ersching J, et al. 2016. PLoS Pathog. 12: 1005698. PubMed
Takeshita S, et al. 2014. J Biol Chem. 289:16699. PubMed

RRID

AB_315553 (BioLegend Cat. No. 510005) AB_2303587 (BioLegend Cat. No. 510006)

Antigen Details

Structure: TNF superfamily; 19 kD (Mammalian)
Distribution: Activated T cells, osteoclasts
Function: Regulates T and dendritic cell interactions, and osteoclast differentiation
Ligand/Receptor: RANK, OPG
Bioactivity: Activates JNK and NF-κB; involved in bone resorption; induces the expression of IL-1, IL-6, IL-12, IL-15 in dendritic cells
Cell Sources: Activated T cells, myeloid bone marrow precursors, stromal fibroblasts, synovial cells, osteoblasts
Cell Targets: Dendritic cells, osteoclasts
Receptors: ODFR, ODAR, TRANCE-R
Cell Type: Osteoclasts, T cells
Biology Area: Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Josien R, et al. 1999. J. Immunol. 162:2562.
3. Takayanagi H, et al. 2000. Nature 408:600.
4. Wong B, et al. 1999. Mol. Cell. 4:1041.
Regulation: Type II integral membrane protein processed by TACE for secretion; upregulated upon TCR engagement
Gene ID: 21943 View all products for this Gene ID
UniProt: View information about CD254 on UniProt.org

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