PE anti-mouse/human Bcl-6 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1836	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Plasmid vector containing cDNA of the Bcl-6 gene

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

This clone is also known as GI191E/A8. Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin fixed, paraffin embedded tissue sections¹, Western blotting¹, and immunoprecipitation¹.

Application References (PubMed link indicates BioLegend citation)

Garcfa JF, et al. 2006. J. Histochem Cytochem. 54:31.

Product Citations

Wilfahrt D, et al. 2021. Elife. 10:. PubMed
Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
Blackburn M, et al. 2015. J Immunol. 195: 3227 - 3236. PubMed
Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed

RRID

AB_2561374 (BioLegend Cat. No. 648303) AB_2561375 (BioLegend Cat. No. 648304)

Antigen Details

Structure: Member of the BTB-POZ zinc finger family, 80 kD, homodimer, 1 BTB (POZ) domain, 6 C2H2-type zinc fingers
Distribution: Germinal center B cells, follicular helper T cells (TFH), B cell lymphomas, breast cancer cells
Function: Transcription factor, master regulator of germinal center reaction
Interaction: BLIMP-1
Cell Type: B cells, Tfh
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Ye BH, et al. 1993. Science 262:747.
2. Baron BW, et al. 1993. P. Natl. Acad. Sci. USA 90:5262.
3. Onizuka T, et al. 1995. Blood 86:28.
4. Johnston RJ, et al. 2009. Science 325:1006.
5. Kitano M, et al. 2011. Immunity 34:961.
6. Baumjohann D, et al. 2011. J. Immunol. 187:2089.
7. Tran TH, et al. 2010. Cancer Res. 70:1711.
Gene ID: 604 View all products for this Gene ID 12053 View all products for this Gene ID
UniProt: View information about Bcl-6 on UniProt.org

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