Ultra-LEAF™ Purified anti-human IFN-β A-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2844	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant HuIFN-β1b

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

WB - Quality tested
ELISA, Neut - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 ?g antibody per 5 mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Neutralization¹: The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IFN-? bioactivity (Cat. No. 514003).

Additional reported applications (for the relevant formats) include: ELISA¹.

Epitope: Human IFN-β site I, core peptide amino acid residues 32-56, AB loop (AB2, AB3)

Application References (PubMed link indicates BioLegend citation)

Redlich PN and Grossberg SE, et al. 1989. J. Immunol. 143:1887. (ELISA, Neut)
Redlich PN, et al. 1991. P. Natl. Acad. Sci. USA 88:4040.
Runkel L, et al. 2001. IFN & Cytokine Res. 21:931.

RRID

AB_2814441 (BioLegend Cat. No. 514005) AB_2814442 (BioLegend Cat. No. 514006)

Antigen Details

Structure: Interferon; contains 187 amino acids with predicted molecular weight 22 kD.
Bioactivity: Non-specific humoral immune responses and immune responses against viral infections, increases expression of MHC class I antigens, blocks expression of MHC class II antigens stimulated by IFN-γ
Cell Sources: Fibroblasts, epithelial cells
Cell Targets: Broad, includes NK cells, T suppressor cells, activated monocytes
Receptors: IFNα/βR (IFNAR-1, IFNAR-2)
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Nagarajan U, 2011. Crit. Rev. Immunol. 31:459.
2. Honda K, et al. 2005. Int. Immunol. 17:1367.
Regulation: Upregulated by viruses, double-stranded RNA, micro-organisms, TNF, IL-1
Gene ID: 3456 View all products for this Gene ID
UniProt: View information about IFN-beta on UniProt.org

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