Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 2.0 ?g per 10⁶ cells in 100 ?l volume or 100 ?l of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy⁵, stimulation of monocytes³, blocking of heterotypic PMN aggregation⁸, and blocking of granulocyte activation¹². This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).

1. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
2. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
3. Rezzonico R, et al. 2001. Blood 97:2932. (Stim)
4. Marsik C, et al. 2003. Shock 20:493. (FC)
5. David A, et al. 2003. J. Leukoc. Biol. 74:551. (IF)
6. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
7. Thurlow LR, et al. 2010. Infect. Immun. 128:1128. (FC) PubMed
8. Jadhav S, et al. 2001. J. Immunol. 167:5986. (Block)
9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
10. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. (FC)
11. Wen T, et al. 2014. J Immunol. 192:5481. (FC) PubMed
12. Sprong T, et al. 2003. Blood 102:3702. (Block)
13. Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
14. Larsson K, et al. 2015. PNAS. PubMed

1. Shukla S, et al. 2021. Nat Chem Biol. 17:784. PubMed
2. Ma L, et al. 2021. Clinical Cancer Research. 27(6):1778-1791. PubMed
3. Zinngrebe J, et al. 2022. EMBO Mol Med. 14:e14901. PubMed
4. Khajuria RK et al. 2018. Cell. 173(1):90-103 . PubMed
5. Wang F, et al. 2021. Genomics Proteomics Bioinformatics. 19:208. PubMed
6. Headland S, et al. 2014. Sci Rep. 4:5237. PubMed
7. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells

1. Stewart M, et al. 1995. Curr?Opin?Cell Biol. 7:690.