GMP PerCP/Cyanine5.5 anti-human CD11b Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100tests	￥ 6336	1

Product Details

Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker?, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

200 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy⁵, stimulation of monocytes³, blocking of heterotypic PMN aggregation⁸, and blocking of granulocyte activation¹². This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).

Application References (PubMed link indicates BioLegend citation)

Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
Barclay N,?et al.?1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
Rezzonico R,?et al.?2001.?Blood?97:2932. (Stim)
Marsik C,?et al.?2003.?Shock?20:493. (FC)
David A,?et al.?2003.?J. Leukoc. Biol.?74:551. (IF)
Charles N,?et al.?2010.?Nat. Med.?16:701. (FC)?PubMed
Thurlow LR,?et al.?2010.?Infect. Immun.?128:1128. (FC)?PubMed
Jadhav S,?et al.?2001.?J. Immunol.?167:5986. (Block)
Yoshino N,?et al.?2000.?Exp. Anim. (Tokyo)?49:97. (FC)
Sestak K,?et al.?2007.?Vet. Immunol. Immunopathol.?119:21. (FC)
Wen T,?et al.?2014.?J Immunol.?192:5481. (FC)?PubMed
Sprong T,?et al.?2003.?Blood?102:3702. (Block)
Cash JL,?et al.?2013.?EMBO Rep.?14:999. (FC)?PubMed
Larsson K,?et al.?2015.?PNAS.?PubMed

RRID

AB_2922524 (BioLegend Cat. No. 260126)

Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: