Brilliant Violet 650™ anti-human CD11b Antib-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2508	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Chimpanzee, Common Marmoset, Pig

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 650? excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy⁵, stimulation of monocytes³, blocking of heterotypic PMN aggregation⁸, and blocking of granulocyte activation¹². This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).

Application References (PubMed link indicates BioLegend citation)

Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
Rezzonico R, et al. 2001. Blood 97:2932. (Stim)
Marsik C, et al. 2003. Shock 20:493. (FC)
David A, et al. 2003. J. Leukoc. Biol. 74:551. (IF)
Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
Thurlow LR, et al. 2010. Infect. Immun. 128:1128. (FC) PubMed
Jadhav S, et al. 2001. J. Immunol. 167:5986. (Block)
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. (FC)
Wen T, et al. 2014. J Immunol. 192:5481. (FC) PubMed
Sprong T, et al. 2003. Blood 102:3702. (Block)
Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
Larsson K, et al. 2015. PNAS. PubMed

Product Citations

Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed

RRID

AB_2562761 (BioLegend Cat. No. 301335) AB_2563793 (BioLegend Cat. No. 301336)

Antigen Details

Structure: Integrin, type I transmembrane glycoprotein, associates with integrin β₂ (CD18), 165-170 kD
Distribution: Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells
Function: Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor: ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type: B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Stewart M, et al. 1995. Curr?Opin?Cell Biol. 7:690.
Gene ID: 3684 View all products for this Gene ID
UniProt: View information about CD11b on UniProt.org

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