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PerCP/Cyanine5.5 anti-human IgD Antibody
产品名称:
PerCP/Cyanine5.5 anti-human IgD Antibody
产品类别:
抗体
产品编号:
348207
产品应用:
348207
[价格]
规格 价格 库存
25tests ¥ 2112 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IgD
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections4?and spatial biology (IBEX)5,6.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Chen K, et al. 2009. Nat. Immunol. 10:889.
  2. Lee CH, et al. 2005. J. Exp. Med. 203:63.
  3. Sutter JA, et al. 2008. Clin. Immunol. 126:282.
  4. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
  5. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  6. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Liu C, et al. 2021. Cell. 184(7):1836-1857.e22. PubMed
  2. Turner JS, et al. 2021. Nature. 596:109. PubMed
  3. Amanat F, et al. 2021. Cell. 184:3936. PubMed
  4. Izmirly AM, et al. 2022. PLoS Pathog. 18:e1009903. PubMed
  5. Parackova Z, et al. 2017. Immunol Lett. 10.1016/j.imlet.2017.04.009. PubMed
  6. James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
  7. Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
  8. Turner JS, et al. 2020. Nature. 586:127. PubMed
  9. Rother M, et al. 2016. J Immunol. 197: 441 - 448. PubMed
  10. Wu S, et al. 2016. Proc Natl Acad Sci U S A. 113: 9816 - 9821. PubMed
  11. van Dongen JJM, et al. 2019. Front Immunol. 10:1271. PubMed
RRID
AB_10642822 (BioLegend Cat. No. 348207) AB_10641706 (BioLegend Cat. No. 348208)

Antigen Details

Structure
Exists in a transmembranal and a soluble form
Distribution

Na?ve B cells

Function
Antigen binding, B cell activation
Interaction
The CD79a/CD79b heterodimer
Cell Type
B cells
Biology Area
Immunology
Antigen References

1. Geisberger R, et al. 2006. Immunology 118:429.
2. Weller S, et al. 2005. Eur. J. Immunol. 35:2789.
3. Brandtzaeg P and Johansen FE. 2005. Immunol. Rev. 206:32.

Gene ID
3495 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org

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