Alexa Fluor® 488 anti-human IgD Antibody
- 产品名称：
- Alexa Fluor® 488 anti-human IgD Antibody
- 产品类别：
- 抗体
- 产品编号：
- 348215
- 产品应用：
- 348215
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1416 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human IgD
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
SB -?Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections4?and spatial biology (IBEX)5,6.
- Additional Product Notes
Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
- Application References
(PubMed link indicates BioLegend citation) -
- Chen K, et al. 2009. Nat. Immunol. 10:889.
- Lee CH, et al. 2005. J. Exp. Med. 203:63.
- Sutter JA, et al. 2008. Clin. Immunol. 126:282.
- Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
- Harrington WE, et al. 2021. Cell Reports Medicine. 2(4):100253. PubMed
- Golovkin A, et al. 2021. Viruses. 13:. PubMed
- Shin JJ, et al. 2022. J Clin Immunol. :. PubMed
- Powell WE, et al. 2018. Diabetologia. 61:1794. PubMed
- Japp AS, et al. 2021. Cell. 184(3):827-839.e14. PubMed
- Kudryavtsev IV, et al. 2021. Curr Issues Mol Biol. 44:194. PubMed
- Yang L, et al. 2020. Genes Dis. 7:128. PubMed
- Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
- Fisher BS, et al. 2020. PLoS One. 15:e0233577. PubMed
- RRID
- AB_11150397 (BioLegend Cat. No. 348215) AB_11150595 (BioLegend Cat. No. 348216)
- Structure
- Exists in a transmembranal and a soluble form
- Distribution
-
Na?ve B cells
- Function
- Antigen binding, B cell activation
- Interaction
- The CD79a/CD79b heterodimer
- Cell Type
- B cells
- Biology Area
- Immunology
- Antigen References
-
1. Geisberger R, et al. 2006. Immunology 118:429.
2. Weller S, et al. 2005. Eur. J. Immunol. 35:2789.
3. Brandtzaeg P and Johansen FE. 2005. Immunol. Rev. 206:32.
- Gene ID
- 3495 View all products for this Gene ID
- UniProt
- View information about IgD on UniProt.org
