Purified anti-mouse CD45 Antibody
- 产品名称：
- Purified anti-mouse CD45 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 147702
- 产品应用：
- 147702
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse lymphoma cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P, IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin embedded sections1 and frozen tissue sections2.
- Application References
(PubMed link indicates BioLegend citation) -
- Kliment C, et al. 2009. J. Mol. Cell Cardiol. 47:730. (IHC)
- Reynolds JM, et al. 2007. J. Immunol. 179:313. (IHC)
- Product Citations
-
- Pilling D, et al. 2022. Respir Res. 23:215. PubMed
- Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
- Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
- Zhang Z, et al. 2018. Nat Med. 24:617. PubMed
- Liu J, et al. 2022. Stem Cell Res Ther. 13:247. PubMed
- RRID
- AB_2563415 (BioLegend Cat. No. 147701) AB_2563416 (BioLegend Cat. No. 147702)
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
- Cell Type
- B cells, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2.?Trowbridge IS and Thomas ML. 1994. Annu. Rev. Immunol. 12:85.?
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
