PerCP/Cyanine5.5 anti-mouse CD45 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1128	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse lymphoma cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin embedded sections¹ and frozen tissue sections².

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Kliment C, et al. 2009. J. Mol. Cell Cardiol. 47:730. (IHC)
Reynolds JM, et al. 2007. J. Immunol. 179:313. (IHC)

Product Citations

Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
Donley DW, et al. 2021. PLOS ONE. 16(5):e0250606. PubMed
Lu P, et al. 2021. Nat Commun. 12:1042. PubMed

RRID

AB_2563537 (BioLegend Cat. No. 147705) AB_2563538 (BioLegend Cat. No. 147706)

Antigen Details

Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type: B cells, Mesenchymal Stem Cells
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2.?Trowbridge IS and Thomas ML. 1994. Annu. Rev. Immunol. 12:85.?
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264 View all products for this Gene ID
UniProt: View information about CD45 on UniProt.org

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