Alexa Fluor® 700 anti-mouse CD45 Antibody
- 产品名称：
- Alexa Fluor® 700 anti-mouse CD45 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 147715
- 产品应用：
- 147715
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse lymphoma cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC -?Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin embedded sections1 and frozen tissue sections2.
- Application References
(PubMed link indicates BioLegend citation) -
- Kliment C, et al. 2009. J. Mol. Cell Cardiol. 47:730. (IHC)
- Reynolds JM, et al. 2007. J. Immunol. 179:313. (IHC)
- Product Citations
-
- Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
- Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
- Ni C, et al. 2022. Nat Commun. 13:5919. PubMed
- Zhou Z, et al. 2022. Life (Basel). 12:. PubMed
- Xiong X et al. 2019. Mol Cell. 75(3):644-660 . PubMed
- Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
- Kung YJ, et al. 2022. J Biomed Sci. 29:57. PubMed
- RRID
- AB_2750448 (BioLegend Cat. No. 147715) AB_2750449 (BioLegend Cat. No. 147716)
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
- Cell Type
- B cells, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2.?Trowbridge IS and Thomas ML. 1994. Annu. Rev. Immunol. 12:85.?
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
