Purified anti-Polyubiquitin (K63-linkage) Antibody-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1212	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Synthetic peptide containing the ubiquitin K-63 isopeptide motif.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

WB - Quality tested
IHC-F, ICC, IP - Reported in the literature, not verified in house, not verified in-house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone HWA4C4 detects Polyubiquitin chains formed by the linkage with Lys63 residue.

It does not cross-react with any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitinylated species.

Application References (PubMed link indicates BioLegend citation)

Dammer E, et al. 2011. J. Biol. Chem. 286:10457. (IHC-F, ICC, WB)
Lin S, et al. 2013. J. Virology 87:11562. (ICC, IP, WB)
Karlsson AB, et al. 2014. Mol. Biol. Cell 25:1355. (WB, ICC)

RRID

AB_2566131 (BioLegend Cat. No. 932201) AB_2566132 (BioLegend Cat. No. 932202)

Antigen Details

Structure: 685 amino acids; 77 kD.
Distribution: Cytoplasmic, nuclear, and extracellular.
Function: Polyubiquitin-C is involved in Poly(A) RNA, protein, and protease binding. The polymeric protein is involved in protein degradation, DNA repair, kinase modification, endocytosis, and cell cycle regulation.
Interaction: Numerous targets including CDK-mediated phosphorylation and removal of CDc6, loss of function of SMAD2/3 in cancer, and signaling by NOTCH1 PEST Domain Mutants in cancer.
Ligand/Receptor: Poly(A) RNA, protein, and proteases.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance, Signal Transduction
Antigen References: 1. Radici L, et al. 2013. Advances in Bioscience and Biotechnology. 4:1057.
2. Walczak H, et al. 2012. Generation and physiological roles of linear ubiquitin chains. BMC Biol. 10:23.
Gene ID: 7316 View all products for this Gene ID
UniProt: View information about Polyubiquitin on UniProt.org

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