Purified anti-Histone H3-Phosphorylated (Ser28) An
- 产品名称：
- Purified anti-Histone H3-Phosphorylated (Ser28) An
- 产品类别：
- 抗体
- 产品编号：
- 641001
- 产品应用：
- 641001
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 of human histone H3.
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store between 2°C and 8°C.
- Application
-
WB - Quality tested
CyTOF?, ICC - Verified
IP, ICFC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
- Application References
(PubMed link indicates BioLegend citation) -
- Hirata A, et al. 2004. J. Histochem. Cytochem. 52:1503.
- Goto H, et al. 1999. J. Biol. Chem. 274:25543.
- Ozawa K. 2008. Cytometry A 73:517.
- Goode NJ, et al. 2014. PLoS Genet. 10:1004323. PubMed
- Product Citations
-
- Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
- de Vargas Roditi L, et al. 2022. Cell Rep Med. 3:100604. PubMed
- Lun XK et al. 2019. Mol Cell. 74(5):1086-1102 . PubMed
- Damond N, et al. 2019. Cell Metab. 29:755. PubMed
- Baran N, et al. 2022. Nat Commun. 13:2801. PubMed
- Tognetti M, et al. 2021. Cell Systems. 12(5):401-418.e12. PubMed
- Godde N, et al. 2014. PLoS Genet. 10:1004323. PubMed
- Bouchard G, et al. 2022. Cancer Res. 82:648. PubMed
- RRID
- AB_1227660 (BioLegend Cat. No. 641001) AB_1227659 (BioLegend Cat. No. 641002)
- Structure
- H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
- Distribution
-
Nucleus
- Function
- H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.
- Interaction
- Two molecules of H3 form a heterotetramer with two molecules of H4.
- Biology Area
- Cell Biology, DNA Repair/Replication, Transcription Factors
- Molecular Family
- Phospho-Proteins
- Antigen References
-
1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545.
2. Goto H, et al. 2002. Genes Cells 7:11.
3. Garcia BA, et al. 2005. Biochemistry 44:13202.
- Regulation
- H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
- Gene ID
- 8290 View all products for this Gene ID
- UniProt
- View information about Phosphorylated Histone H3 on UniProt.org
