PerCP/Cyanine5.5 anti-Histone H3-Phosphorylated (S
- 产品名称：
- PerCP/Cyanine5.5 anti-Histone H3-Phosphorylated (S
- 产品类别：
- 抗体
- 产品编号：
- 641013
- 产品应用：
- 641013
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2112 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 of human histone H3.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Hirata A, et al. 2004. J. Histochem. Cytochem. 52:1503.
- Goto H, et al. 1999. J. Biol. Chem. 274:25543.
- Ozawa K. 2008. Cytometry A 73:517.
- Goode NJ, et al. 2014. PLoS Genet. 10:1004323. PubMed
- RRID
- AB_2565891 (BioLegend Cat. No. 641013) AB_2565892 (BioLegend Cat. No. 641014)
- Structure
- H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
- Distribution
-
Nucleus
- Function
- H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.
- Interaction
- Two molecules of H3 form a heterotetramer with two molecules of H4.
- Biology Area
- Cell Biology, DNA Repair/Replication, Transcription Factors
- Molecular Family
- Phospho-Proteins
- Antigen References
-
1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545.
2. Goto H, et al. 2002. Genes Cells 7:11.
3. Garcia BA, et al. 2005. Biochemistry 44:13202.
- Regulation
- H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
- Gene ID
- 8290 View all products for this Gene ID
- UniProt
- View information about Phosphorylated Histone H3 on UniProt.org
