Ultra-LEAF™ Purified anti-human CD284 (TLR4)-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2844	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Guinea Pig

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Ba/F3 cell line expressing human TLR4

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-F, ICC, WB, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections⁴, immunofluorescence microscopy⁶, Western blotting¹⁰, and in vitro blocking of LPS-induced cytokine production^2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 312813 & 312814).?

Application References (PubMed link indicates BioLegend citation)

Skimazu R, et al. 1999. J. Exp. Med. 189:1777.
Wang R, et al. 2003. Hybrid Hybridomics 22:357. (Block)
Wang JE, et al. 2001. Infect. Immun. 69:2402. (Block)
Ishihara S, et al. 2004 J. Immunol. 173:1406. (IHC)
Kawahara T, et al. 2001 Infect. Immun. 69:4382.
Jiang Q, et al. 2000. J. Immunol. 165:3541. (IF)
Sugawara S, et al. 2001. Infect. Immun. 69:4951. (Block)
Chavakis E, et al. 2007. Circ. Res. 100:204. PubMed
Bhattacharyya S, et al. 2007. Am. J. Physiol. Gastrointest Liver Physiol. doi:10.1152/ajpgi.00149. (Block) PubMed
Baumgarten G, et al. 2001. J. Infectious. Dis. 183:1617.

Product Citations

Lee Y, et al. 2022. Front Immunol. 12:780210. PubMed

RRID

AB_2814162 (BioLegend Cat. No. 312813) AB_2561485 (BioLegend Cat. No. 312814)

Antigen Details

Structure: Type I transmembrane protein, Toll-like receptor family/IL-1R superfamily, 110 kD
Distribution: Monocytes/macrophages, endothelial cells, B cells and granulocytes
Function: Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
Ligand/Receptor: Bacterial LPS
Cell Type: B cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: 1. Skimazu R, et al. 1999. J. Exp. Med. 189:1777.
Gene ID: 7099 View all products for this Gene ID
UniProt: View information about CD284 on UniProt.org

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