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PE anti-human CD284 (TLR4) Antibody
产品名称：
PE anti-human CD284 (TLR4) Antibody
产品类别：
抗体
产品编号：
312805
产品应用：
312805
[价格]
规格 价格 库存
25tests ￥ 1464 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Guinea Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ba/F3 cell line expressing human TLR4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4, immunofluorescence microscopy6, Western blotting10, and in vitro blocking of LPS-induced cytokine production2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 312813 & 312814).?

Application References

(PubMed link indicates BioLegend citation)
  1. Skimazu R, et al. 1999. J. Exp. Med. 189:1777.
  2. Wang R, et al. 2003. Hybrid Hybridomics 22:357. (Block)
  3. Wang JE, et al. 2001. Infect. Immun. 69:2402. (Block)
  4. Ishihara S, et al. 2004 J. Immunol. 173:1406. (IHC)
  5. Kawahara T, et al. 2001 Infect. Immun. 69:4382.
  6. Jiang Q, et al. 2000. J. Immunol. 165:3541. (IF)
  7. Sugawara S, et al. 2001. Infect. Immun. 69:4951. (Block)
  8. Chavakis E, et al. 2007. Circ. Res. 100:204. PubMed
  9. Bhattacharyya S, et al. 2007. Am. J. Physiol. Gastrointest Liver Physiol. doi:10.1152/ajpgi.00149. (Block) PubMed
  10. Baumgarten G, et al. 2001. J. Infectious. Dis. 183:1617.
Product Citations
  1. Shen Y, et al. 2021. Cell Stress Chaperones. 26:937. PubMed
  2. Li Y, et al. 2019. J Biol Chem. 294:2744. PubMed
  3. Hong S, et al. 2014. Mol Immunol. 57:284. PubMed
  4. Metwally H, et al. 2020. Sci Signal. :13. PubMed
  5. Bunt S, et al. 2009. J Leukoc Biol. 85:996. PubMed
  6. Tatematsu M, et al. 2016. J Immunol. 196: 3865 - 3876. PubMed
  7. Sp N, et al. 2021. Life (Basel). 11: . PubMed
  8. Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed
  9. Munawara U, et al. 2021. Immun Ageing. 18:29. PubMed
  10. Tidu F, et al. 2021. iScience. 24(6):102683. PubMed
  11. Bessich J, et al. 2013. J Immunol. 191:378. PubMed
  12. Luo J, et al. 2019. Int J Immunopathol Pharmacol. 33:2058738419828890. PubMed
  13. Hidaka M, et al. 2013. Eur J Pharmacol. 721:305. PubMed
  14. Wentworth J, et al. 2010. Diabetes. 59:1648. PubMed
  15. Lee GR, et al. 2021. JCI Insight. 6:. PubMed
  16. Kang DY, et al. 2021. Mol Med Rep. 24:. PubMed
  17. Huizinga R, et al. 2013. J Immunol. 191:5636. PubMed
  18. Yin X, et al. 2010. Biochem Biophys Res Commun. 397:232. PubMed
  19. Kaufman T, et al. 2017. Clin Immunol. 10.1016/j.clim.2017.08.014. PubMed
  20. Masat E, et al. 2015. Sci Rep. 5: 14847. PubMed
RRID
AB_314954 (BioLegend Cat. No. 312805) AB_2205002 (BioLegend Cat. No. 312806)

Antigen Details

Structure
Type I transmembrane protein, Toll-like receptor family/IL-1R superfamily, 110 kD
Distribution

Monocytes/macrophages, endothelial cells, B cells and granulocytes

Function
Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
Ligand/Receptor
Bacterial LPS
Cell Type
B cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Skimazu R, et al. 1999. J. Exp. Med. 189:1777.

Gene ID
7099 View all products for this Gene ID
UniProt
View information about CD284 on UniProt.org

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