Brilliant Violet 421™ anti-human CD284 (TLR4-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2580	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Guinea Pig

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Ba/F3 cell line expressing human TLR4

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections⁴, immunofluorescence microscopy⁶, Western blotting¹⁰, and in vitro blocking of LPS-induced cytokine production^2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 312813 & 312814).?

Application References (PubMed link indicates BioLegend citation)

Skimazu R, et al. 1999. J. Exp. Med. 189:1777.
Wang R, et al. 2003. Hybrid Hybridomics 22:357. (Block)
Wang JE, et al. 2001. Infect. Immun. 69:2402. (Block)
Ishihara S, et al. 2004 J. Immunol. 173:1406. (IHC)
Kawahara T, et al. 2001 Infect. Immun. 69:4382.
Jiang Q, et al. 2000. J. Immunol. 165:3541. (IF)
Sugawara S, et al. 2001. Infect. Immun. 69:4951. (Block)
Chavakis E, et al. 2007. Circ. Res. 100:204. PubMed
Bhattacharyya S, et al. 2007. Am. J. Physiol. Gastrointest Liver Physiol. doi:10.1152/ajpgi.00149. (Block) PubMed
Baumgarten G, et al. 2001. J. Infectious. Dis. 183:1617.

Product Citations

Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
Hadadi E, et al. 2016. Sci Rep. 6:39035. PubMed
Schaper F, et al. 2016. Rheumatology. 55(12):2260-2270. PubMed

RRID

AB_10896054 (BioLegend Cat. No. 312811)

Antigen Details

Structure: Type I transmembrane protein, Toll-like receptor family/IL-1R superfamily, 110 kD
Distribution: Monocytes/macrophages, endothelial cells, B cells and granulocytes
Function: Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
Ligand/Receptor: Bacterial LPS
Cell Type: B cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: 1. Skimazu R, et al. 1999. J. Exp. Med. 189:1777.
Gene ID: 7099 View all products for this Gene ID
UniProt: View information about CD284 on UniProt.org

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