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FITC anti-human CD158 (KIR2DL1/S1/S3/S5) Antibody
产品名称:
FITC anti-human CD158 (KIR2DL1/S1/S3/S5) Antibody
产品类别:
抗体
产品编号:
339503
产品应用:
339503
[价格]
规格 价格 库存
25tests ¥ 1212 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human NK cell clone LB2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

mAb HP-MA4 reacts with KIR2DL1 (CD158a), KIR2DS1 (CD158h), KIR2DS3, and KIR2DS5 (CD158g).

Additional reported applications include: inhibits NK cell mediated cytotoxicity and immunoprecipitation.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Application References

(PubMed link indicates BioLegend citation)
  1. De Miguel M and M. Lopez-Botet. 2002. Inmunologia. 21:187
  2. Goodridge JP, et al. 2013. J. Immunol. 191:3553. PubMed
Product Citations
  1. Hydes T, et al. 2017. Immun Inflamm Dis. 10.1002/iid3.190. PubMed
  2. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  3. Guo M, et al. 2021. Front Oncol. 11:632540. PubMed
  4. Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed
  5. Huang RS, et al. 2021. Curr Protoc. 1:e246. PubMed
  6. Lim D, et al. 2014. J Immunol. 193:5065. PubMed
  7. Reches A, et al. 2016. J Immunol. 196: 4967 - 4976. PubMed
  8. Harris LD, et al. 2020. Front Cell Infect Microbiol. 10:120. PubMed
  9. Goodridge J, et al. 2013. J Immunol. 191:3553. PubMed
  10. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
RRID
AB_1501076 (BioLegend Cat. No. 339503) AB_2130378 (BioLegend Cat. No. 339504)

Antigen Details

Structure
Contains two Ig-like extracellular domains, 58/50 kD
Distribution

Subset of NK cells and a small subset of CD8+ T cells

Function
Inhibits NK cell function, play a role in self-tolerance.
Ligand/Receptor
HLA-C
Cell Type
NK cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication

Gene ID
3802 View all products for this Gene ID 3810 View all products for this Gene ID 3808 View all products for this Gene ID 3806 View all products for this Gene ID
UniProt
View information about CD158 on UniProt.org

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