Ultra-LEAF™ Purified anti-mouse/rat CD29 Ant-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2844	1

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Purified mouse VLA-4 (α₄β₁, CD49d/CD29)

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-F, IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemistry⁴ of acetone-fixed frozen sections, in vitro blocking of the adhesion of mouse tumor cell lines to extracellular matrix proteins and in vitro inhibition of T cell proliferative responses¹, and in vivo inhibition of neutrophil migration². The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102235 and 102236).

Application References (PubMed link indicates BioLegend citation)

Noto K, et al. 1995. Int. Immunol. 7:835.
Ridger VC, et al. 2001. J. Immunol. 166:3484.
Jia W, et al. 2005. Blood 106:3854.PubMed
Economopoulou M, et al. 2005. Blood 106:3831.
Lawson BR, et al. 2007. J. Immunol. 178:5366.
Eisenmann KM, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M703243200.PubMed
Hayashi Y, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 294:G778. PubMed
Kim DT, et al. 2008. Blood 111:2929.PubMed
Hayashi Y, et al. 2008. J Pharmacol Exp Ther. 326:523. PubMed
Carlson TR, et al. 2008. Development. 135:2193. PubMed
Sangaletti S, et al. 2008. Cancer Res. 68:9050. (Block) PubMed
Baker CM, et al. 2012. PNAS. PubMed.
Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed

Product Citations

Freeman SA, et al. 2020. Science. 367:301. PubMed
Hwang B, et al. 2021. Nat Methods. 18:903. PubMed

RRID

AB_2813927 (BioLegend Cat. No. 102235) AB_2813928 (BioLegend Cat. No. 102236)

Antigen Details

Structure: Integrin family, 130 kD
Distribution: Leukocytes, endothelial cells, smooth muscle, epithelial cells
Function: Adhesion
Ligand/Receptor: Extracellular matrix
Cell Type: Embryonic Stem Cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal Stem Cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Noto K, et al. 1995. Int. Immunol. 7:835.
2. Springer TA. 1990. Nature 346:425.
Gene ID: 16412 View all products for this Gene ID 24511 View all products for this Gene ID
UniProt: View information about CD29 on UniProt.org

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