PerCP/Cyanine5.5 anti-mouse/rat CD29 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1344	1

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Purified mouse VLA-4 (α₄β₁, CD49d/CD29)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemistry⁴ of acetone-fixed frozen sections, in vitro blocking of the adhesion of mouse tumor cell lines to extracellular matrix proteins and in vitro inhibition of T cell proliferative responses¹, and in vivo inhibition of neutrophil migration². The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102235 and 102236).

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Noto K, et al. 1995. Int. Immunol. 7:835.
Ridger VC, et al. 2001. J. Immunol. 166:3484.
Jia W, et al. 2005. Blood 106:3854.PubMed
Economopoulou M, et al. 2005. Blood 106:3831.
Lawson BR, et al. 2007. J. Immunol. 178:5366.
Eisenmann KM, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M703243200.PubMed
Hayashi Y, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 294:G778. PubMed
Kim DT, et al. 2008. Blood 111:2929.PubMed
Hayashi Y, et al. 2008. J Pharmacol Exp Ther. 326:523. PubMed
Carlson TR, et al. 2008. Development. 135:2193. PubMed
Sangaletti S, et al. 2008. Cancer Res. 68:9050. (Block) PubMed
Baker CM, et al. 2012. PNAS. PubMed.
Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed

Product Citations

Chato-Astrain J, et al. 2019. Front Cell Neurosci. 12:501. PubMed

RRID

AB_2572078 (BioLegend Cat. No. 102227) AB_2572079 (BioLegend Cat. No. 102228)

Antigen Details

Structure: Integrin family, 130 kD
Distribution: Leukocytes, endothelial cells, smooth muscle, epithelial cells
Function: Adhesion
Ligand/Receptor: Extracellular matrix
Cell Type: Embryonic Stem Cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal Stem Cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Noto K, et al. 1995. Int. Immunol. 7:835.
2. Springer TA. 1990. Nature 346:425.
Gene ID: 16412 View all products for this Gene ID 24511 View all products for this Gene ID
UniProt: View information about CD29 on UniProt.org

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