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FITC anti-mouse/rat CD29 Antibody
产品名称:
FITC anti-mouse/rat CD29 Antibody
产品类别:
抗体
产品编号:
102205
产品应用:
102205
[价格]
规格 价格 库存
50µg ¥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Purified mouse VLA-4 (α4β1, CD49d/CD29)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry4 of acetone-fixed frozen sections, in vitro blocking of the adhesion of mouse tumor cell lines to extracellular matrix proteins and in vitro inhibition of T cell proliferative responses1, and in vivo inhibition of neutrophil migration2. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102235 and 102236).

Application References

(PubMed link indicates BioLegend citation)
  1. Noto K, et al. 1995. Int. Immunol. 7:835.
  2. Ridger VC, et al. 2001. J. Immunol. 166:3484.
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  5. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  6. Eisenmann KM, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M703243200.PubMed
  7. Hayashi Y, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 294:G778. PubMed
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  12. Baker CM, et al. 2012. PNAS. PubMed.
  13. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
Product Citations
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  2. Yang M, et al. 2020. Cell Prolif. 53:e12784. PubMed
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  7. Li L, et al. 2017. Stem Cell Research & Therapy . 10.1186/s13287-017-0565-7. PubMed
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  13. Takahashi M, et al. 2009. Am J Physiol Heart Circ Physiol. 298:415. PubMed
  14. Zhao J, et al. 2018. Stem Cell Reports. 10:180. PubMed
  15. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  16. Sun P, et al. 2021. J Mammary Gland Biol Neoplasia. 26:377. PubMed
  17. Hu Y, et al. 2021. Bioact Mater. 2905:6. PubMed
  18. Chou PR, et al. 2020. Int J Med Sci. 17:354. PubMed
  19. Tong L, et al. 2016. Sci Rep. 6:21642. PubMed
  20. Gu B, et al. 2009. J Cell Biol. 185:811. PubMed
  21. McNeil M, et al. 2021. J Mammary Gland Biol Neoplasia. 26:357. PubMed
  22. Manupati K, et al. 2022. Cancers (Basel). 14:. PubMed
  23. Xu X, et al. 2022. EMBO Rep. 23:e53509. PubMed
  24. Cai W, et al. 2013. J Cell Sci. 126:2877. PubMed
  25. Watzlawik J, et al. 2010. Glia. 58:1782. PubMed
  26. Li CJ, et al. 2018. J Clin Invest. 128:5251. PubMed
  27. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  28. Hou J, et al. 2017. Mol Med Rep. 16:1502. PubMed
  29. Yang Y, et al. 2021. Cell Reports. 34(10):108822. PubMed
  30. Yu JM, et al. 2019. Nat Commun. 4.388888889. PubMed
  31. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  32. Liu F, et al. 2021. Cell Reports. 35(10):109225. PubMed
  33. Bao Z, et al. 2020. Ann Transplant. 25:e921287. PubMed
  34. Felce JH, et al. 2018. Sci Signal. 11:eaat0756. PubMed
  35. Ge Q, et al. 2018. Mol Med Rep. 17:1667. PubMed
  36. Zhao W, et al. 2022. Bioeng Transl Med. 7:e10303. PubMed
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RRID
AB_312882 (BioLegend Cat. No. 102205) AB_312883 (BioLegend Cat. No. 102206)

Antigen Details

Structure
Integrin family, 130 kD
Distribution

Leukocytes, endothelial cells, smooth muscle, epithelial cells

Function
Adhesion
Ligand/Receptor
Extracellular matrix
Cell Type
Embryonic Stem Cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Noto K, et al. 1995. Int. Immunol. 7:835.
2. Springer TA. 1990. Nature 346:425.

Gene ID
16412 View all products for this Gene ID 24511 View all products for this Gene ID
UniProt
View information about CD29 on UniProt.org

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