Ultra-LEAF™ Purified anti-mouse/rat CD49e An
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse/rat CD49e An
- 产品类别：
- 抗体
- 产品编号：
- 103909
- 产品应用：
- 103909
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Affinity-Purified mouse VLA-5 protein (α5β1, CD49e/CD29)
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IP, Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vivo inhibition of fibronectin receptor (VLA-5) functions and in vitro blocking of CD49e mediated interactions 1,2. The Ultra-LEAF? Purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103909 & 103910).
- Application References
(PubMed link indicates BioLegend citation) -
- Yasuda M, et al. 1995. Int. Immunol. 7:251. (IP Block)
- Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
- Lin JS, et al. 2010. J. Leukoc. Biol. 88:95. PubMed
- Product Citations
-
- Inverso D, et al. 2021. Developmental Cell. 56(11):1677-1693.e10. PubMed
- He C, et al. 2022. JCI Insight. 7:. PubMed
- RRID
- AB_2832320 (BioLegend Cat. No. 103909) AB_2832321 (BioLegend Cat. No. 103910)
- Structure
- Integrin family, associates with integrin β1, 135 kD
- Distribution
-
Activated T cells, mast cells
- Function
- Adhesion, costimulation
- Ligand/Receptor
- Fibronectin
- Cell Type
- Mast cells, Mesenchymal Stem Cells, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1994 Blood Cells 20:25.
3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365.
- Gene ID
- 16402 View all products for this Gene ID 315346 View all products for this Gene ID
- UniProt
- View information about CD49e on UniProt.org
