PE anti-mouse/rat CD49e Antibody
- 产品名称：
- PE anti-mouse/rat CD49e Antibody
- 产品类别：
- 抗体
- 产品编号：
- 103905
- 产品应用：
- 103905
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Affinity-Purified mouse VLA-5 protein (α5β1, CD49e/CD29)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vivo inhibition of fibronectin receptor (VLA-5) functions and in vitro blocking of CD49e mediated interactions 1,2. The Ultra-LEAF? Purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103909 & 103910).
- Application References
(PubMed link indicates BioLegend citation) -
- Yasuda M, et al. 1995. Int. Immunol. 7:251. (IP Block)
- Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
- Lin JS, et al. 2010. J. Leukoc. Biol. 88:95. PubMed
- Product Citations
-
- Filippi A, et al. 2020. Cell Transplant. 29:963689720946277. PubMed
- Wang C, et al. 2009. Biomaterials. 30:2259. PubMed
- RRID
- AB_2128165 (BioLegend Cat. No. 103905) AB_2265017 (BioLegend Cat. No. 103906)
- Structure
- Integrin family, associates with integrin β1, 135 kD
- Distribution
-
Activated T cells, mast cells
- Function
- Adhesion, costimulation
- Ligand/Receptor
- Fibronectin
- Cell Type
- Mast cells, Mesenchymal Stem Cells, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1994 Blood Cells 20:25.
3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365.
- Gene ID
- 16402 View all products for this Gene ID 315346 View all products for this Gene ID
- UniProt
- View information about CD49e on UniProt.org
