Biotin anti-mouse/rat CD49e Antibody -上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 3240	1

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Affinity-Purified mouse VLA-5 protein (α₅β₁, CD49e/CD29)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vivo inhibition of fibronectin receptor (VLA-5) functions and in vitro blocking of CD49e mediated interactions ^1,2. The Ultra-LEAF? Purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103909 & 103910).

Application References (PubMed link indicates BioLegend citation)

Yasuda M, et al. 1995. Int. Immunol. 7:251. (IP Block)
Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
Lin JS, et al. 2010. J. Leukoc. Biol. 88:95. PubMed

RRID

AB_2128166 (BioLegend Cat. No. 103904)

Antigen Details

Structure: Integrin family, associates with integrin β₁, 135 kD
Distribution: Activated T cells, mast cells
Function: Adhesion, costimulation
Ligand/Receptor: Fibronectin
Cell Type: Mast cells, Mesenchymal Stem Cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1994 Blood Cells 20:25.
3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365.
Gene ID: 16402 View all products for this Gene ID 315346 View all products for this Gene ID
UniProt: View information about CD49e on UniProt.org

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