PE/Cyanine7 anti-mouse CD49b Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1344	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Mouse colon carcinoma cell line Colon26

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunofluorescence on frozen sections⁴, and blocking of cell adhesion^1-3. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103525 & 103526).

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (Block, IP)
Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
Arase H, et al. 2001. J. Immunol. 167:1141. (Block)
Zhang Z, et al. 2008. Blood 111:1980. (IF)

Product Citations

Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed
Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed

RRID

AB_2566102 (BioLegend Cat. No. 103517) AB_2566103 (BioLegend Cat. No. 103518)

Antigen Details

Structure: Integrin family, associates with CD29 (β₁ integrin), 150 kD
Distribution: NK cells, subset splenic CD4⁺ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, platelets
Function: Adhesion, lymphocyte activation
Ligand/Receptor: Collagen, laminin, fibronectin
Cell Type: Epithelial cells, Lymphocytes, NK cells, NKT cells, Platelets, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Sasaki K, et al. 2003. Int. Immunol. 15:701.
3. Inoue O, et al. 2003. J. Cell Biol. 160:769.
Gene ID: 16398 View all products for this Gene ID
UniProt: View information about CD49b on UniProt.org

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