PE anti-mouse CD49b Antibody
- 产品名称：
- PE anti-mouse CD49b Antibody
- 产品类别：
- 抗体
- 产品编号：
- 103506
- 产品应用：
- 103506
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse colon carcinoma cell line Colon26
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence on frozen sections4, and blocking of cell adhesion1-3. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103525 & 103526).
- Application References
(PubMed link indicates BioLegend citation) -
- Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (Block, IP)
- Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
- Arase H, et al. 2001. J. Immunol. 167:1141. (Block)
- Zhang Z, et al. 2008. Blood 111:1980. (IF)
- Product Citations
-
- Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
- Mirshahi F, et al. 2022. Cell Rep. 38:110454. PubMed
- Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
- Qian P et al. 2018. Cell stem cell. 22(5):740-754 . PubMed
- Gozgit JM, et al. 2021. Cancer Cell. :. PubMed
- , et al. 2021. Eur J Immunol. 51:2708. PubMed
- Kim SJ, et al. 2021. Front Immunol. 12:772859. PubMed
- Giraddi R, et al. 2015. Nat Commun. 6: 8487. PubMed
- Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
- Del Barco Barrantes I, et al. 2018. Stem Cell Reports. 10:257. PubMed
- Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
- Englund JI, et al. 2021. Development. 148:. PubMed
- Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed
- Seong J, et al. 2018. Development. 145:14. PubMed
- RRID
- AB_313029 (BioLegend Cat. No. 103506)
- Structure
- Integrin family, associates with CD29 (β1 integrin), 150 kD
- Distribution
-
NK cells, subset splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, platelets
- Function
- Adhesion, lymphocyte activation
- Ligand/Receptor
- Collagen, laminin, fibronectin
- Cell Type
- Epithelial cells, Lymphocytes, NK cells, NKT cells, Platelets, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Sasaki K, et al. 2003. Int. Immunol. 15:701.
3. Inoue O, et al. 2003. J. Cell Biol. 160:769.
- Gene ID
- 16398 View all products for this Gene ID
- UniProt
- View information about CD49b on UniProt.org
