Alexa Fluor® 488 anti-mouse CD49b Antibody
- 产品名称：
- Alexa Fluor® 488 anti-mouse CD49b Antibody
- 产品类别：
- 抗体
- 产品编号：
- 103510
- 产品应用：
- 103510
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse colon carcinoma cell line Colon26
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence on frozen sections4, and blocking of cell adhesion1-3. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103525 & 103526).
- Application References
(PubMed link indicates BioLegend citation) -
- Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (Block, IP)
- Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
- Arase H, et al. 2001. J. Immunol. 167:1141. (Block)
- Zhang Z, et al. 2008. Blood 111:1980. (IF)
- Product Citations
-
- Nakajima T, et al. 2021. J Exp Med. 218:. PubMed
- Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
- Walens A, et al. 2019. Elife. 8:e43653. PubMed
- Dietschmann A, et al. 2020. Eur J Immunol. 50:1044. PubMed
- RRID
- AB_492851 (BioLegend Cat. No. 103510)
- Structure
- Integrin family, associates with CD29 (β1 integrin), 150 kD
- Distribution
-
NK cells, subset splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, platelets
- Function
- Adhesion, lymphocyte activation
- Ligand/Receptor
- Collagen, laminin, fibronectin
- Cell Type
- Epithelial cells, Lymphocytes, NK cells, NKT cells, Platelets, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Sasaki K, et al. 2003. Int. Immunol. 15:701.
3. Inoue O, et al. 2003. J. Cell Biol. 160:769.
- Gene ID
- 16398 View all products for this Gene ID
- UniProt
- View information about CD49b on UniProt.org
