Purified anti-mouse Notch 3 Antibody
- 产品名称：
- Purified anti-mouse Notch 3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 130502
- 产品应用：
- 130502
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Notch 3-Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- Moriyama Y, et al. 2008. Int J Immunology 20:763
- Ghosh S, et al. 2011. J. Biol Chem. 286:22678. PubMed.
- Romano RA, et al. 2012. Development 139:772. PubMed.
- Product Citations
-
- Ghosh S, et al. 2011. J Biol Chem. 286:22678. PubMed
- Romano R, et al. 2012. Development. 139:772. PubMed
- Zhang H et al. 2017. eLife. 6 pii: e30126. PubMed
- RRID
- AB_1227735 (BioLegend Cat. No. 130502)
- Structure
- Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
- Distribution
-
Notch 3 is primarily expressed in vascular smooth muscle cells, also found in proliferating neuroepithelium.
- Function
- Critical role in the structural integrity of distal resistance artieries. Mutations in the Notch 3 receptor on vascular smooth muscles cells is associated with an inherited neurodegenerative disease CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopthy)
- Ligand/Receptor
- Jag1,2; Delta 1,2
- Cell Type
- Epithelial cells
- Biology Area
- Immunology
- Antigen References
-
1. Ehebauer ME, et al. 2006. Biochem J 392:13
2. Shimizu K, et al. 2000. Mol Cell Biology 20:18
3. Tanigaki K, et al. 2007. Nature Immunol 8:451
4. Bellavia D, et al. 2008. 27:5092
5. Louvi A, et al. Dev Neurosci. 2006. 28:5
- Gene ID
- 18131 View all products for this Gene ID
- UniProt
- View information about Notch 3 on UniProt.org
