Alexa Fluor® 647 anti-mouse Notch 3 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-mouse Notch 3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 130511
- 产品应用：
- 130511
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Notch 3-Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Moriyama Y, et al. 2008. Int J Immunology 20:763
- Product Citations
-
- Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
- RRID
- AB_2151249 (BioLegend Cat. No. 130511) AB_2151248 (BioLegend Cat. No. 130512)
- Structure
- Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
- Distribution
- Notch 3 is primarily expressed in vascular smooth muscle cells, also found in proliferating neuroepithelium.
- Function
- Critical role in the structural integrity of distal resistance artieries. Mutations in the Notch 3 receptor on vascular smooth muscles cells is associated with an inherited neurodegenerative disease CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopthy)
- Ligand/Receptor
- Jag1,2; Delta 1,2
- Cell Type
- Epithelial cells
- Biology Area
- Immunology
- Antigen References
- 1. Ehebauer ME et al. 2006. Biochem J 392:13
2. Shimizu K et al. 2000. Mol Cell Biology 20:18
3. Tanigaki K et al. 2007. Nature Immunol 8:451
4. Bellavia D et al. 2008. 27:5092
5. Louvi A et al. Dev Neurosci. 2006. 28:5 - Gene ID
- 18131 View all products for this Gene ID
- UniProt
- View information about Notch 3 on UniProt.org
