APC anti-mouse Notch 2 Antibody
- 产品名称：
- APC anti-mouse Notch 2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 130713
- 产品应用：
- 130713
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Notch 2-Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Moriyama Y et al. 2008. Int J Immunology 20:763
- Product Citations
-
- Hombrink P, et al. 2016. Nat Immunol. 17:1467-1478. PubMed
- Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
- Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
- RRID
- AB_2153643 (BioLegend Cat. No. 130713) AB_2153642 (BioLegend Cat. No. 130714)
- Structure
- Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
- Distribution
- Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart. In spleen, Notch 2 is reported to be expressed primarily on B cells.
- Function
- Regulate broad spectrum of various cell lineage commitment process, including myogenesis, neurogenesis, gliogenesis and lymphocyte development. Mutation of Notch2 can cause a rare genetic disorder, Alagille syndrome, with multiple defects in liver, heart and other systems.
- Ligand/Receptor
- Jag1,2; Delta 1,4
- Cell Type
- B cells, Neural Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Antigen References
-
1. Ehebauer ME, et al. 2006. Biochem J 392:13
2. Shimizu K, et al. 2000. Mol Cell Biology 20:18
3. Tanigaki K, et al. 2007. Nature Immunol 8:451
4. Kraman M, et al. 2005. FASEB J. 19:1311
- Gene ID
- 18129 View all products for this Gene ID
- UniProt
- View information about Notch 2 on UniProt.org
