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首页 > 抗体蛋白 > 抗体
FITC anti-mouse CD48 Antibody
产品名称：
FITC anti-mouse CD48 Antibody
产品类别：
抗体
产品编号：
103403
产品应用：
103403
[价格]
规格 价格 库存
50µg ￥ 1272 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse T lymphoma MBL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
  2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
Product Citations
  1. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  2. Lengefeld J, et al. 2021. Sci Adv. 7:eabk0271. PubMed
  3. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  4. Maltby S, et al. 2014. J Immunol. 193:4072. PubMed
  5. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  6. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  7. Guo B, et al. 2018. Nat Med. 24:360. PubMed
  8. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  9. Ganuza M, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.04.006. PubMed
  10. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  11. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  12. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  13. Georgievski A, et al. 2022. Cell Death Dis. 13:337. PubMed
  14. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  15. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  16. Dawar S, et al. 2016. Cell Death Dis. 7:e2509. PubMed
  17. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  18. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  19. Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed
  20. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  21. Nakamura-Ishizu A, et al. 2012. Blood. 119:5429. PubMed
  22. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  23. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  24. Koide S, et al. 2022. iScience. 25:103603. PubMed
  25. Artinger E, et al. 2013. Proc Natl Acad Sci U S A. 110:12000. PubMed
  26. Theocharides A, et al. 2012. J Exp Med. 209:1883. PubMed
  27. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  28. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  29. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  30. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  31. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  32. Lv K, et al. 2021. Cell Stem Cell. 28(7):1275-1290.e9. PubMed
  33. Chou S, et al. 2013. Exp Hematol. 41:479. PubMed
  34. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  35. Abdel Malik R, et al. 2017. Circ Res. 120:99. PubMed
RRID
AB_313018 (BioLegend Cat. No. 103403) AB_313019 (BioLegend Cat. No. 103404)

Antigen Details

Structure
Ig superfamily, 45 kD
Distribution

T cells and B cells, monocytes/macrophages

Function
Adhesion, T cell recognition
Ligand/Receptor
CD2, CD244
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
12506 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org

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