Brilliant Violet 711™ anti-mouse CD48 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 3708	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Mouse T lymphoma MBL-2

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711? under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711? excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, costimulation of T cell proliferation^1,2, blocking of CD48-CD2 interaction¹, and inhibition of CTL activity and graft rejection^1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)

Product Citations

Bonnet C, et al. 2021. iScience. 24:103399. PubMed
Yoshimi A, et al. 2019. Nature. 574:273. PubMed
Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
Tang W, et al. 2021. Front Cell Dev Biol. 9:728057. PubMed
Fielding C, et al. 2022. Nat Commun. 13:543. PubMed

RRID

AB_2650824 (BioLegend Cat. No. 103439)

Antigen Details

Structure: Ig superfamily, 45 kD
Distribution: T cells and B cells, monocytes/macrophages
Function: Adhesion, T cell recognition
Ligand/Receptor: CD2, CD244
Cell Type: B cells, Macrophages, Monocytes, T cells
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.
Gene ID: 12506 View all products for this Gene ID
UniProt: View information about CD48 on UniProt.org

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