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APC/Cyanine7 anti-mouse CD48 Antibody
产品名称：
APC/Cyanine7 anti-mouse CD48 Antibody
产品类别：
抗体
产品编号：
103431
产品应用：
103431
[价格]
规格 价格 库存
25µg ￥ 1548 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse T lymphoma MBL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/?g).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
  2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
Product Citations
  1. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  2. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  3. Naka K, et al. 2015. Nat Commun. 6: 8039. PubMed
  4. Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed
  5. Naka K, et al. 2020. Nat Commun. 3.709027778. PubMed
  6. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  7. Sykes DB et al. 2016. Cell. 167(1):171-186 . PubMed
  8. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  9. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  10. Li CC, et al. 2022. Nat Commun. 13:346. PubMed
  11. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  12. De Koninck M, et al. 2020. Cell Reports. 32(6):108014. PubMed
  13. Simon M, et al. 2019. Cell Metab. 29:871. PubMed
  14. Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed
  15. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  16. Morales Torres C, et al. 2020. Nat Commun. 11:1792. PubMed
  17. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  18. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  19. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  20. Giannini S, et al. 2020. Nat Commun. 0.705555556. PubMed
  21. Brandi P, et al. 2022. Cell Rep. 38:110184. PubMed
  22. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  23. Rebekka K Schneider et al. 2017. Cell stem cell. 20(6):785-800 . PubMed
  24. Passaro D et al. 2017. Cancer cell. 32(3):324-341 . PubMed
  25. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  26. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  27. Yang T, et al. 2022. Nat Commun. 13:4464. PubMed
  28. Mistry JJ, et al. 2021. Nat Commun. 12:7130. PubMed
  29. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  30. Young IC, et al. 2021. Cell Rep. 37:110036. PubMed
  31. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  32. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  33. Lv K, et al. 2021. Cell Stem Cell. 28(7):1275-1290.e9. PubMed
  34. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
RRID
AB_2561462 (BioLegend Cat. No. 103431) AB_2561463 (BioLegend Cat. No. 103432)

Antigen Details

Structure
Ig superfamily, 45 kD
Distribution

T cells and B cells, monocytes/macrophages

Function
Adhesion, T cell recognition
Ligand/Receptor
CD2, CD244
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
12506 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org

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