Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, costimulation of T cell proliferation^1,2, blocking of CD48-CD2 interaction¹, and inhibition of CTL activity and graft rejection^1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/?g).

1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)

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T cells and B cells, monocytes/macrophages

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