Alexa Fluor® 647 anti-mouse CD48 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2748	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Mouse T lymphoma MBL-2

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, costimulation of T cell proliferation^1,2, blocking of CD48-CD2 interaction¹, and inhibition of CTL activity and graft rejection^1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)

Product Citations

Ho TT, et al. 2021. J Exp Med. 218:. PubMed
Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed
Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
Alvarez S, et al. 2015. Nat Commun. 6: 8548. PubMed
Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed

RRID

AB_571987 (BioLegend Cat. No. 103416)

Antigen Details

Structure: Ig superfamily, 45 kD
Distribution: T cells and B cells, monocytes/macrophages
Function: Adhesion, T cell recognition
Ligand/Receptor: CD2, CD244
Cell Type: B cells, Macrophages, Monocytes, T cells
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.
Gene ID: 12506 View all products for this Gene ID
UniProt: View information about CD48 on UniProt.org

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