Alexa Fluor® 647 anti-mouse CD40 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1212	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

(BALB/c x NZB)F₁ mouse-derived lymphoma WEHI 231

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD40 ligand binding¹, stimulation of mouse B cell and dendritic cell proliferation^2-6,9, and immunohistochemistry⁷ of acetone-fixed frozen sections. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102908).

Application References (PubMed link indicates BioLegend citation)

Kawano T, et al. 1997. Science 278:1626. (Block)
Kaneko Y, et al. 1996. Eur. J. Immunol. 26:3061. (Costim)
Kashiwada M, et al. 1996. Eur. J. Immunol. 26:1451. (Costim)
Inaba M, et al. 1995. Eur. J. Immunol. 25:1244. (Costim)
Akiba H, et al. 1999. J. Immunol. 162:7058. (Costim)
Ridge JP, et al. 1998. Nature 393:474. (Costim)
Morelli AE, et al. 2000. Transplantation 69:2647. (IHC)
Ma XT, et al. 2006. Cancer Research 66:1169.
Yu X, et al. 2006. J. Biol. Chem. 281:15505. (Costim)
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed

Product Citations

Misharin A, et al. 2012. J Leukoc Biol. 92:325. PubMed
Wang S, et al. 2018. Biomaterials. 187:28:00. PubMed
Klimmeck D, et al. 2012. Mol Cell Proteomics. 11:286. PubMed
Wang Y, et al. 2021. Cell Rep. 35:108948. PubMed
Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed

RRID

AB_492855 (BioLegend Cat. No. 102911) AB_492854 (BioLegend Cat. No. 102912)

Antigen Details

Structure: TNFR superfamily, 48 kD
Distribution: B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34⁺ hematopoietic progenitors
Function: Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
Ligand/Receptor: CD40L (CD154)
Cell Type: B cells, Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Macrophages
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.
Gene ID: 21939 View all products for this Gene ID
UniProt: View information about CD40 on UniProt.org

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