PerCP/Cyanine5.5 anti-mouse CD34 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD34 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 128607
- 产品应用：
- 128607
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse CD34 transfected BHK cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and 564 nm and a maximun emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The HM34 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Product Citations
-
- Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
- Shi Y, et al. 2020. Med Sci Monit. 26:e923328. PubMed
- Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
- Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
- Ding P, et al. 2022. Bone Res. 10:42. PubMed
- Mistry JJ, et al. 2021. Nat Commun. 12:7130. PubMed
- Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
- Zheng X, et al. 2015. Cell Rep. 13:1505-1518. PubMed
- Oppezzo A, et al. 2020. J Clin Invest. 130:1377. PubMed
- Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
- RRID
- AB_1279222 (BioLegend Cat. No. 128607) AB_2260023 (BioLegend Cat. No. 128608)
- Structure
- Type I membrane protein, highly glycosylated, 75-120 kd, two isoforms reported.
- Distribution
-
Hematopoietic progenitors, brain, testis, endothelial cells
- Function
- Possible adhesion molecules thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Present carbohydrate ligands to selectins.
- Ligand/Receptor
- L-selectin, and others.
- Cell Type
- Endothelial cells, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Garlanda C, et al. 1997. Eur J Cell Biol 73:368
2. Brown J, et al. 1991. Int Immunol 3:175
- Gene ID
- 12490 View all products for this Gene ID
- UniProt
- View information about CD34 on UniProt.org
