Alexa Fluor® 488 anti-mouse CD195 (CCR5) Antib
- 产品名称：
- Alexa Fluor® 488 anti-mouse CD195 (CCR5) Antib
- 产品类别：
- 抗体
- 产品编号：
- 107008
- 产品应用：
- 107008
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
CCR5 is expressed at low density on activated cells. For successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107004) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).
- Application References
(PubMed link indicates BioLegend citation) -
- Mao A, et al. 2005. J. Immunol. 175:5146. (FC) PubMed
- Ishida Y, et al. 2007. Am J Pathol.170:843.(FC) PubMed
- Zeiser Z, et al. 2008. Blood 111:453. (FC) PubMed
- Sharma R, et al. 2009. J. Immunol.. 183:3212 (FC) PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- Product Citations
-
- Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
- Zhang T, et al. 2022. Oncogene. . PubMed
- Ishida Y, et al. 2007. Am J Pathol . 170:843. PubMed
- Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
- RRID
- AB_528756 (BioLegend Cat. No. 107008)
- Structure
- β-chemokine receptor, 45 kD
- Distribution
-
Macrophages, T cell subset, heart, spleen, liver
- Function
- Lymphocyte chemotaxis and transendothelial migration during inflammation, signaling through seven transmembrane-spanning G proteins
- Ligand/Receptor
- RANTES, MCP-1, MIP-1α, and MIP-1β
- Cell Type
- Dendritic cells, Macrophages, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Napolitano M, et al. 1990. J. Exp. Med. 172:285.
3. Meyer A, et al. 1996. J. Biol. Chem. 271:14445.
4. Boring, et al. 1996. J. Biol. Chem. 271:7551.
- Gene ID
- 12774 View all products for this Gene ID
- UniProt
- View information about CD195 on UniProt.org
