Ultra-LEAF™ Purified anti-human CD229 (Ly-9)
- 产品名称：
- Ultra-LEAF™ Purified anti-human CD229 (Ly-9)
- 产品类别：
- 抗体
- 产品编号：
- 326109
- 产品应用：
- 326109
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- 300.19 cell line transfected with the CD229 full-length DNA
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IP, ICC, FA - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, and immunocytochemistry3. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 326109 & 326110).
- Application References
(PubMed link indicates BioLegend citation) -
- de la Fuente MA, et al. 2001. Blood 97:3513.
- Martin M, et al. 2005. J. Immunol. 174:5977.
- Romero X, et al. 2005. J. Immunol. 174:7033.
- RRID
- AB_2832637 (BioLegend Cat. No. 326109) AB_2832638 (BioLegend Cat. No. 326110)
- Structure
- 100-120 kD glycoprotein, SLAM family, CD2 subset of Ig superfamily
- Distribution
-
T cells, B cells, NK cells, thymocytes
- Function
- Homophilic adhesion, T cell activation, Th2 cell polarization
- Ligand/Receptor
- CD229
- Cell Type
- B cells, NK cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Sandrin MS, et al. 1996. Immunogenetics 43:13.
2. de la Fuente MA, et al. 2001. Blood 97:3513.
3. Romero X, et al. 2005. J. Immunol. 174:7033.
- Gene ID
- 4063 View all products for this Gene ID
- UniProt
- View information about CD229 on UniProt.org
