Ultra-LEAF™ Purified anti-mouse CD275 (B7-H2-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse B7-RP1 transfected cell line

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?L?volume or 100 ?L?of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of ligand binding^1,2. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107405) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107404) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 107410)?(Endotoxin <0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Iwai H, et al. 2003. J. Immunol. 171:2848. (Block)
Kohyama M, et al. 2004. P. Natl. Acad. Sci. USA 101:4192. (Block)
Zhang P, et al. 2007. J.Immunol. 178:474. PubMed
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed

Product Citations

Verma M, et al. 2021. J Exp Med. 218:. PubMed
Rivera CA, et al. 2022. Immunity. 55:129. PubMed

RRID

AB_2832361 (BioLegend Cat. No. 107411) AB_11149485 (BioLegend Cat. No. 107410) AB_2832362 (BioLegend Cat. No. 107412) AB_2832363 (BioLegend Cat. No. 107413) AB_2832364 (BioLegend Cat. No. 107414) AB_2832365 (BioLegend Cat. No. 107415)

Antigen Details

Structure: B7 Immunoglobulin superfamily, approximately 40 kD
Distribution: Isoform 1 has highest expression in lymphoid tissues, splenic B-cells, T-cells, dendritic cells and macrophages; lower levels in many non-lymphoid tissues. The expression of isoform 2 is restricted to heart, spleen, and kidney.
Function: Binds to ICOS. Co-stimulates T cell responses including proliferation and cytokine secretion. Stimulates B cell proliferation and differentiation to plasma cells.
Ligand/Receptor: ICOS
Cell Type: B cells, Dendritic cells, Lymphocytes, Macrophages, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Yoshinaga SK, et al. 1999. Nature 402:827.
2. Ling V, et al. 2000. J. Immunol. 164:1653.
3. Ling V, et al. 2001. J. Immunol. 164:1653.
Gene ID: 50723 View all products for this Gene ID
UniProt: View information about CD275 on UniProt.org

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