PerCP anti-mouse Ly-6C Antibody
- 产品名称：
- PerCP anti-mouse Ly-6C Antibody
- 产品类别：
- 抗体
- 产品编号：
- 128027
- 产品应用：
- 128027
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- L3 cloned CTL cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume.? It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Clone HK1.4 does not block the binding of clone RB6-8C58.
Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.
- Application References
(PubMed link indicates BioLegend citation) -
- Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
- Herold KC, et al. 1990. Diabetes 39:815. (Activ)
- Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
- Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
- Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
- Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
- Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
- Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
- Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
- Product Citations
-
- D?britz J, et al. 2016. Sci Rep. 6:20584. PubMed
- Chinta KC et al. 2018. Cell reports. 25(7):1938-1952 . PubMed
- Chao CC, et al. 2020. Cell. 179(7):1483-1498.e22.. PubMed
- Tsiganov E, et al. 2014. J Immunol. 192:4718. PubMed
- Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
- Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
- Zheng J, et al. 2020. bioRxiv. . PubMed
- Wheeler MA, et al. 2019. Cell. 176:581. PubMed
- Zheng J, et al. 2020. Nature. . PubMed
- Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
- Buzzelli JN, et al. 2019. Am J Physiol Gastrointest Liver Physiol. 316:G251. PubMed
- Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
- Molgora M, et al. 2020. Cell. 182:886. PubMed
- Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed
- Wheeler MA, et al. 2020. Nature. 578:593. PubMed
- Davis FM, et al. 2021. J Exp Med. 218:. PubMed
- Goddery EN, et al. 2021. Front Immunol. 12:726421. PubMed
- RRID
- AB_10900235 (BioLegend Cat. No. 128027) AB_10897805 (BioLegend Cat. No. 128028)
- Structure
- 14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
- Distribution
-
Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.
- Cell Type
- Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.
- Gene ID
- 17067 View all products for this Gene ID
- UniProt
- View information about Ly-6C on UniProt.org
