PE/Dazzle™ 594 anti-mouse Ly-6C Antibody
- 产品名称：
- PE/Dazzle™ 594 anti-mouse Ly-6C Antibody
- 产品类别：
- 抗体
- 产品编号：
- 128043
- 产品应用：
- 128043
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- L3 cloned CTL cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Clone HK1.4 does not block the binding of clone RB6-8C58.
Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.
- Application References
(PubMed link indicates BioLegend citation) -
- Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
- Herold KC, et al. 1990. Diabetes 39:815. (Activ)
- Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
- Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
- Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
- Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
- Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
- Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
- Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
- Product Citations
-
- Wang X, et al. 2019. Cell Res. 29:787. PubMed
- Putnam NE, et al. 2019. PLoS Pathog. 15:e1007744. PubMed
- Chikina AS, et al. 2020. Cell. 183:411. PubMed
- Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
- Tuit S, et al. 2019. Cell Rep. 29:1221. PubMed
- An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed
- Soto-Díaz K, et al. 2021. Front Immunol. 12:734349. PubMed
- Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
- Zhang X, et al. 2021. Mol Cancer Res. 19:1076. PubMed
- Trivedi S, et al. 2020. Elife. 9:00. PubMed
- RRID
- AB_2566576 (BioLegend Cat. No. 128043) AB_2566577 (BioLegend Cat. No. 128044)
- Structure
- 14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
- Distribution
-
Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.
- Cell Type
- Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.
- Gene ID
- 17067 View all products for this Gene ID
- UniProt
- View information about Ly-6C on UniProt.org
