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PE/Cyanine7 anti-mouse Ly-6C Antibody
产品名称:
PE/Cyanine7 anti-mouse Ly-6C Antibody
产品类别:
抗体
产品编号:
128017
产品应用:
128017
[价格]
规格 价格 库存
25µg ¥ 1488 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Oliver AJ, et al. 2020. Oncoimmunology. 9:1802979. PubMed
  2. Bernasconi R, et al. 2021. EMBO Mol Med. :e14392. PubMed
  3. Drobek A, et al. 2015. J Immunol. 195: 3416 - 3426. PubMed
  4. Moyat M, et al. 2015. Infect Immun . 83: 4217 - 4228. PubMed
  5. Dudek M, et al. 2015. Mucosal Immunol. . PubMed
  6. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  7. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  8. Stein K, et al. 2016. J Leukoc Biol. 99: 231 - 239. PubMed
  9. Choi YY, et al. 2021. Cell Death Dis. 12:826. PubMed
  10. Zas?ona Z, et al. 2014. J Immunol. 193:4245. PubMed
  11. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  12. Gon?alves NP, et al. 2017. J Neuroinflammation. 14:115. PubMed
  13. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  14. Albarrán–Juárez J, et al. 2018. J Exp Med. 215:2655. PubMed
  15. Stolp B, et al. 2022. Cell Rep. 38:110387. PubMed
  16. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  17. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  18. Kleinholz CL, et al. 2021. Sci Rep. 11:15071. PubMed
  19. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  20. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  21. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  22. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  23. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  24. Diao N, et al. 2016. Sci Rep. 6:34672. PubMed
  25. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  26. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  27. Palathingal Bava E, et al. 2022. JCI Insight. 7:. PubMed
  28. Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed
  29. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
  30. Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed
  31. Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
  32. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  33. Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed
  34. Shami A, et al. 2015. Sci Rep. 5: 13904. PubMed
  35. Iizasa E, et al. 2021. Nat Commun. 12:2299. PubMed
  36. Geng S, et al. 2021. JCI Insight. 6:. PubMed
  37. Warheit-Niemi HI, et al. 2022. JCI Insight. 7:. PubMed
  38. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  39. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
  40. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  41. Molina M, et al. 2015. PLoS One. 10:127262. PubMed
  42. Tang CC, et al. 2021. eLife. 10:00. PubMed
  43. Zhang F, et al. 2020. EMBO Mol Med. e12034:12. PubMed
  44. Wu W, et al. 2018. J Am Soc Nephrol. 29:168. PubMed
  45. Pascual–García M, et al. 2019. Nat Commun. 10:2416. PubMed
  46. Momin N, et al. 2022. Nat Commun. 13:109. PubMed
  47. Pradhan K, et al. 2021. Front Immunol. 12:778830. PubMed
  48. Garg M, et al. 2021. Cell Reports. 34(6):108736. PubMed
  49. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  50. Niknam S, et al. 2018. Clin Cancer Res. 24:5735. PubMed
  51. Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed
  52. Gurusamy M, et al. 2021. Nat Commun. 12:6798. PubMed
  53. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  54. Albarrán-Juárez J, et al. 2016. Atherosclerosis. 251:445-53. PubMed
  55. Saha S, et al. 2016. Nat Commun. 7:13096. PubMed
  56. Runyan CE, et al. 2020. Aging Cell. 19:00. PubMed
  57. Zhou G, et al. 2019. FASEB J. 33:11396. PubMed
  58. Steiger S, et al. 2015. J Immunol. 195: 5495 - 5502. PubMed
  59. Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed
  60. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  61. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  62. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  63. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  64. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  65. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  66. Penke LR, et al. 2020. Life Sci Alliance. 3:. PubMed
  67. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  68. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  69. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  70. Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed
  71. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  72. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  73. Feng L, et al. 2022. Signal Transduct Target Ther. 7:21. PubMed
  74. Wang X, et al. 2016. J Immunol. 197(12):4750-4761. PubMed
  75. Weinstock NI, et al. 2020. Neuron. 107(1):65-81. PubMed
  76. van Geffen C, et al. 2021. Front Immunol. 12:695933. PubMed
  77. Johnson RM, et al. 2021. Front Immunol. 11:614697. PubMed
  78. Yuan C, et al. 2015. Biochem Biophys Res Commun. 464: 249-255. PubMed
  79. Rasid O, et al. 2020. Cell Reports. 29(12):3933-3945.e3.. PubMed
  80. Pradhan K, et al. 2021. J Immunol. 206:2980. PubMed
  81. Zhang T, et al. 2020. JCI Insight. 5:00. PubMed
  82. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  83. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  84. Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed
  85. Rodriguez-Tirado C, et al. 2022. Cancers (Basel). 14:. PubMed
  86. Liu SS, et al. 2021. Immunity. :. PubMed
  87. Nakayama Y, et al. 2020. Proc Natl Acad Sci U S A. 117:14365. PubMed
  88. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
  89. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  90. Lau A, et al. 2022. Sci Adv. 8:eabm0142. PubMed
  91. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  92. Clement M, et al. 2014. Proc Natl Acad Sci U S A. 111:1101. PubMed
  93. Ang QY, et al. 2020. Cell. 181(6):1263-1275.e16. PubMed
  94. Zhang J, et al. 2020. Cell Metabolism. 31(6):1136-1153. PubMed
  95. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
  96. Geng S, et al. 2016. Nat Commun. 7:13436. PubMed
  97. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  98. Syed I et al. 2018. Cell metabolism. 27(2):419-427 . PubMed
RRID
AB_1732093 (BioLegend Cat. No. 128017) AB_1732082 (BioLegend Cat. No. 128018)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

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