Pacific Blue™ anti-mouse Ly-6C Antibody
- 产品名称：
- Pacific Blue™ anti-mouse Ly-6C Antibody
- 产品类别：
- 抗体
- 产品编号：
- 128013
- 产品应用：
- 128013
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- L3 cloned CTL cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Clone HK1.4 does not block the binding of clone RB6-8C58.
Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.
- Application References
(PubMed link indicates BioLegend citation) -
- Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
- Herold KC, et al. 1990. Diabetes 39:815. (Activ)
- Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
- Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
- Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
- Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
- Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
- Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
- Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
- Product Citations
-
- Yang C, et al. 2022. Nat Commun. 13:3426. PubMed
- Orberg E, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.53. PubMed
- Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
- Frieler RA, et al. 2017. Exp Neurol. 298:104. PubMed
- Mathur R, et al. 2019. Mucosal Immunol. 12:612. PubMed
- Zhang R, et al. 2019. Cell Rep. 28:2647. PubMed
- Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
- Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
- Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed
- Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
- Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
- Bourn JR, et al. 2021. Cancer Lett. 503:75. PubMed
- Lebratti T, et al. 2021. eLife. 10:00. PubMed
- Spees A, et al. 2014. Infect Immun . 82:1692. PubMed
- Mooney J, et al. 2015. Sci Rep. 5: 14603. PubMed
- Li J, et al. 2019. JCI Insight. 5. PubMed
- Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
- Hoffmann J, et al. 2014. PLoS One. 9:98456. PubMed
- Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
- Ding P, et al. 2022. Bone Res. 10:42. PubMed
- Jin C, et al. 2019. Cell. 176:998. PubMed
- Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
- Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
- Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
- Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed
- Cunha LD et al. 2018. Cell. 175(2):429-441 . PubMed
- Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
- Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
- Li S, et al. 2019. Front Neurol. 1.036805556. PubMed
- Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
- Muchenditsi A, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 313:G39. PubMed
- Manríquez V, et al. 2021. Nat Commun. 12:4547. PubMed
- Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
- Gharib S, et al. 2014. J Leukoc Biol. 95:9. PubMed
- Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed
- Kusnadi A, et al. 2020. Immunity. 51(2):241-257. PubMed
- Ho A, et al. 2011. J Immunol. 187:6011. PubMed
- Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
- RRID
- AB_1732090 (BioLegend Cat. No. 128013) AB_1732079 (BioLegend Cat. No. 128014)
- Structure
- 14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
- Distribution
-
Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.
- Cell Type
- Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.
- Gene ID
- 17067 View all products for this Gene ID
- UniProt
- View information about Ly-6C on UniProt.org
